Methods for kinetic and thermodynamic analysis of aminoacyl-tRNA synthetases
نویسندگان
چکیده
منابع مشابه
Aminoacyl-tRNA synthetases database
Aminoacyl-tRNA synthetases (AARSs) are at the center of the question of the origin of life. They constitute a family of enzymes integrating the two levels of cellular organization: nucleic acids and proteins. AARSs arose early in evolution and are believed to be a group of ancient proteins. They are responsible for attaching amino acid residues to their cognate tRNA molecules, which is the firs...
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Previous proteomic analyses have shown that aminoacyl-tRNA synthetases (aaRSs) in many organisms can be modified by acetylation of lysine (Lys). In this present study, leucyl-tRNA synthetase and arginyl-tRNA synthetase from Escherichia coli (EcLeuRS and EcArgRS) were overexpressed and purified, and found to be acetylated on Lys residues by mass spectrometry (MS). Glutamine (Gln) scanning mutage...
متن کاملAminoacyl-tRNA synthetases database Y2K
The aminoacyl-tRNA synthetases (AARS) are a diverse group of enzymes that ensure the fidelity of transfer of genetic information from DNA into protein. They catalyse the attachment of amino acids to transfer RNAs and thereby establish the rules of the genetic code by virtue of matching the nucleotide triplet of the anticodon with its cognate amino acid. Currently, 818 AARS primary structures ha...
متن کاملAminoacyl-tRNA Synthetases and Self-acylating Ribozymes
The aminoacyl-tRNA synthetases (aaRS) are at the heart of modern translation, catalyzing the accurate biosynthesis of aminoacyl-tRNAs (aa-tRNAs), the immediate precursors for encoded peptides. However, the first catalysts that made aa-RNAs for coded protein synthesis probably appeared long before any protein aaRS, to serve a preexisting translation system (see below). It presently seems likely ...
متن کاملAminoacyl-tRNA synthetases in medicine and disease
Aminoacyl-tRNA synthetases (ARSs) are essential and ubiquitous 'house-keeping' enzymes responsible for charging amino acids to their cognate tRNAs and providing the substrates for global protein synthesis. Recent studies have revealed a role of multiple ARSs in pathology, and their potential use as pharmacological targets and therapeutic reagents. The ongoing discovery of genetic mutations in h...
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ژورنال
عنوان ژورنال: Methods
سال: 2008
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2007.09.007